Hodnocení cytokinového imunobarvení u ovariálních novotvarů a endometriomů


Authors: Rosekeila Simões Nomelini 1,2 ;  Priscylla Kelliny Aparecida Antão 1 ;  Millena Prata Jammal 1 ;  Cid Almeida De Lima 1 ;  Paula Carolina Arvelos Crispim 1 ;  Agrimaldo Martins-Filho 1 ;  Renata Margarida Etchebehere 3 ;  Eddie Fernando Candido Murta 1
Authors‘ workplace: Laboratory of Applied Sciences for Women (LaCam)/ Department of Gynecology and Obstetrics, Federal University of Triângulo Mineiro, Uberaba-MG, Brazil 1;  Graduate Program in Gynecology and Obstetrics of the Ribeirão Preto Medical School of the University of São Paulo (FMRP-USP), Ribeirão Preto, São Paulo, Brazil 2;  Surgical Pathology Service, Federal University of Triângulo Mineiro, Uberaba-MG, Brazil 3
Published in: Ceska Gynekol 2024; 89(5): 377-384
Category: Original Article
doi: https://doi.org/10.48095/cccg2024377

Overview

Cíl: Cílem naší studie bylo kvantifikovat a porovnat imunobarvení IL-2, IL-5, IL-6, IL-8 a TNFa ve tkáni endometriomu, nenádorových nádorech, benigních novotvarech a maligních novotvarech vaječníků. Materiál a metody: Studie se zúčastnilo 90 pacientek; bylo dia gnostikováno 15 nenádorových lézí ovaria, 28 ovariálních benigních novotvarů, 28 ovariálních maligních novotvarů a 19 ovariálních endometriomů. Imunohistochemie byla provedena pro cytokiny IL-2, IL-5, IL-6, IL-8 a TNFa a jejich koncentrace byly v těchto skupinách porovnány. Použili jsme Fisherův exaktní test, podle něhož jsou významné hodnoty p < 0,05. Výsledky: Imunobarvení epitelu na přítomnost IL-5 a IL-8 je silnější u endometriomů než u karcinomu vaječníků (p hodnoty 0,0046 a 0,0149). Stromální imunobarvení TNFa, IL-5, IL-6 a IL8 je silnější u endometriomů než u rakoviny vaječníků (p hodnoty 0,0008; < 0,0001; 0,0003 a 0,0006). Závěr: Silnější imunobarvení ně kte rých cytokinů u endometriomů ve srovnání s karcinomem ovaria odráží zánětlivou a imunitní odpověď, která by mohla být budoucím cílem nových objevů o infiltrativním chování endometriózy.

Klíčová slova:

cytokiny – Endometrióza – imunologie – ovariální novotvary


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ORCID authors
R. Simões Nomelin 0000-0001-6591-7619
P. K. Aparecia Antão 0000-0002-4294-5892
M. Prata Jammal 0000-0003-2693-8817
C. Almeida de Lima 0000-0002-6926-558X
P. C. Arvelos Crispim 0000-0003-2637-2462
A. Martins-Filho 0000-0002-2519-5583
R. M. Etchebehere 0000-0002-2241-5966
E. F. Candido Murta 0000-0003-4014-1345
Submitted/Doručeno: 28. 4. 2024
Accepted/Přijato: 30. 4. 2024
Prof. Rosekeila Simões Nomelini, MD
Laboratory of Applied Sciences for Women (LaCam)
Department of Gynecology and Obstetrics
Federal University of Triângulo Mineiro
Av. Getúlio Guaritá 214, Bairro Abadia
Uberaga-MG 38025-440
Brazil
rosekeila@terra.com.br
Labels
Paediatric gynaecology Gynaecology and obstetrics Reproduction medicine

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Czech Gynaecology

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2024 Issue 5

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