Biochemical aspects of fetal hypoxia

Authors: K. Biringer 1;  J. Danko 1;  K. Dókuš 1;  K. Maťašová 2;  M. Zibolen 2;  R. Pullmann 3
Authors‘ workplace: Gynekologicko-pôrodnícka klinika JLF UK a UNM, Martin, prednosta prof. MUDr. J. Danko, CSc. 1;  Neonatologická klinika JLF UK a UNM, Martin, prednosta prof. MUDr. M. Zibolen, CSc. 2;  Ústav klinickej biochémie JLF UK a UNM, Martin, prednosta prof. MUDr. RNDr. R. Pullmann, PhD. 3
Published in: Ceska Gynekol 2011; 76(4): 285-291


To evaluate validity of biochemical diagnostic methods of fetal hypoxia.

A case-control study.

Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic.

We included 67 patients, and they were retrospectively divided into group of controls (n=36), and studied group (n=31) according to pH in umbilical artery (UA) <7.15. Acid-base parameters were assessed with Rapidlab 248, Bayer Healthcare LLC, East Walpole, USA. We determined criterion for metabolic acidosis (MAC) as pH UA <7.15, resp. base deficit (BD) UA >12 mmol/l. Postpartal lactate concentration in umbilical vein (UV) and UA was determined with lactatemeter Accutrend Lactate, Roche Diagnostics, Switzerland. Quantitative assessment of fetal human protein S100B was provided with ELISA (Sangtec 100 ELISA, DiaSorin Inc., Stillwater, Minnesota, USA). Fetal erythropoietin concentration in UV was examined with immunoenzymatic assessment Access EPO (Beckman Coulter, Inc., Fullerton, CA, USA). Statistics: histograms, Kolmogorov-Smirnov test, Mann-Whitney test, Spearman’s rho; statistical significance: p<0.05, Receiver Operating Characteristic curves, Area Under the Curve.

The best correlation was between fetal acid-base parameters and lactate in UA (p<0.0005). Significant correlation was between EPO in UV, and protein S100B in UV (p<0.05). EPO in UV significantly correlated with lactate in UA (p<0.05). Correlation between EPO in UV and protein S100B was not significant. According to ROC curves in prediction of fetal hypoxia, we found an excellent accuracy (AUC>0.9) for lactate in UA, good accuracy (AUC>0.7) had EPO in UV. Results for protein S100B were not significant. The highest sensitivity had EPO in UV, while the highest specificity has had lactate in UA.

An indisputable evidence of labor management quality is the fetal metabolic status. On the basis of our results, the suitable clinical markers are lactate and EPO, in addition to acid-base parameters.

Key words:
fetal hypoxia, acid-base, lactate, erythropoietin, protein S100B.


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Paediatric gynaecology Gynaecology and obstetrics Reproduction medicine
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