HE4 a biomarker of ovarian cancer

Authors: J. Presl 1 ;  R. Kučera 2;  O. Topolčan 2;  Z. Novotný 1;  J. Vrzalová 2;  R. Fuchsová 2;  L. Betincová 1;  Z. Rokyta 1
Authors‘ workplace: Gynekologicko-porodnická klinika LF UK a FN Plzeň, přednosta doc. MUDr. Z. Novotný, CSc. 1;  Imunoanalytická laboratoř LF UK a FN Plzeň, přednosta prof. MUDr. O. Topolčan, CSc. 2
Published in: Ceska Gynekol 2012; 77(5): 445-449


Verification of the importance of determination of HE4 and calculation of ROMA index for increasing the efficiency of diagnosis of ovarian cancer in a population of Czech women.

Prospective study.

Department of Gynaecology and Obstetrics, Faculty Hospital in Pilsen.

In the period from 06/24/2010 to 12/01/2011 was at the Department of Gynaecology and Obstetrics, University Hospital Pilsen examined 552 patients with abnormalities in the pelvis. Patients were divided into two groups. There were 30 women with histologically confirmed malignant ovarian tumors. Another 522 women had benign findings. According to the levels of FSH were women in both groups divided into premenopausal and postmenopausal. At all women were measured CA 125, HE4 and FSH. HE4 and CA125 were determined using the chemiluminescent device Architect 1000 (Abbott, USA), FSH chemiluminescent method on the device DXI 800 (Beckman Coulter, USA). At all premenopausal women was calculated ROMA1 index and at all postmenopausal women ROMA2 index. SAS statistical software 9.2 were used for all statistical calculations.

The highest diagnostic efficiency was achieved by a combination of HE4 and CA125 markers with the calculation ROMA2 index for postmenopausal women. In determining of menopausal status according to the values of FSH cut-off for menopause 40 IU/L and cut-off at 26.4% for ROMA2 reaches ROMA2 sensitivity of 92.3%, specificity of 88.5% and PV- of 99.3%. If we reduce the cut-off for laboratory diagnosis of menopause using FSH at 22 IU/L, and cut-off for ROMA2 was 26.3% reaches ROMA2 sensitivity of 95.2%, specificity of 87.8% and PV- of 99.5%.

HE4 in combination with CA125 and current ROMA index calculation is a suitable methodology to improve the detection of ovarian cancer.

Key words:
HE4, CA125, ROMA index, ovarian cancer, menopausal status.


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Paediatric gynaecology Gynaecology and obstetrics Reproduction medicine

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